Anti-Sorbitol Dehydrogenase antibody (ab241303)
Key features and details
- Rabbit polyclonal to Sorbitol Dehydrogenase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sorbitol Dehydrogenase antibody
See all Sorbitol Dehydrogenase primary antibodies -
Description
Rabbit polyclonal to Sorbitol Dehydrogenase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Sorbitol Dehydrogenase aa 307-357. The exact sequence is proprietary. (NP_003095.2).
Database link: Q00796 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Sorbitol Dehydrogenase immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Sorbitol Dehydrogenase antibody (ab241303)All lanes : Anti-Sorbitol Dehydrogenase antibody (ab241303) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Sorbitol Dehydrogenase antibody (ab241303)Sorbitol Dehydrogenase was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab241303 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241303 at 0.4 µg/ml.
Lane 1: ab241303 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 10 seconds.
