Anti-Smg1 antibody (ab80600)
Key features and details
- Rabbit polyclonal to Smg1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Smg1 antibody
See all Smg1 primary antibodies -
Description
Rabbit polyclonal to Smg1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Smg1 aa 1250-1300. The exact sequence is proprietary.
Database link: NP_055907.3 -
Positive control
- HeLa cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smg1 antibody (ab80600)All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg
Lane 2 : Whole HeLa cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 410 kDa
Observed band size: 410 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Chemiluminescence with exposure times of 30 seconds -
Immunoprecipitation - Anti-Smg1 antibody (ab80600)Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins
