Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smg1 antibody (ab30916)

Key features and details

  • Rabbit polyclonal to Smg1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Smg1 antibody
    See all Smg1 primary antibodies

  • Description

    Rabbit polyclonal to Smg1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 3600 - C-terminus of Human Smg1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab31816.)

  • Positive control

    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at 1:200 (See Abreview Feb 20 2007).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 340 kDa (predicted molecular weight: 340 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.

    • Tissue specificity

      Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • 61E3.4 antibody
        • ATX antibody
        • hSMG-1 antibody
        • hSMG1 antibody
        • KIAA0421 antibody
        • Lambda interacting protein antibody
        • Lambda-interacting protein antibody
        • Lambda/iota protein kinase C interacting protein antibody
        • Lambda/iota protein kinase C-interacting protein antibody
        • LIP antibody
        • Phosphatidylinositol 3 kinase related kinase antibody
        • Phosphatidylinositol 3 kinase related protein kinase antibody
        • PI 3 kinase related kinase SMG 1 antibody
        • Serine/threonine protein kinase SMG1 antibody
        • Serine/threonine-protein kinase SMG1 antibody
        • SMG-1 antibody
        • smg1 antibody
        • SMG1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Smg1 antibody (ab30916)
        Western blot - Anti-Smg1 antibody (ab30916)
        All lanes : Anti-Smg1 antibody (ab30916) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
        Lane 2 : Jurkat whole cell lysate (ab7899) at 10 µg
        Lane 3 : A-431 whole cell lysate (ab7909) at 20 µg

        Secondary
        All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

        Performed under reducing conditions.

        Predicted band size: 340 kDa
        Observed band size: 340 kDa



        The band at 340kDa is likely to represent isoform 3 of Smg1. The immunogen is present in all three isoforms of Smg1.   

      • Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)This image is courtesy of an Abreview submitted by Dr Kirk McManus
        ab30916 (1/200) staining Smg1 by ICC/IF in assynchronous HeLa Cells (green). Cells were countersatined with DAPI (red) in order to show the nucleus. Secondary antibody: Goat anti-Rabbit conjugated to Cy3®. Please refer to abreview for further details.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        ICC/IF image of ab30916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30916, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml

      References (4)

      Publishing research using ab30916? Please let us know so that we can cite the reference in this datasheet.

      ab30916 has been referenced in 4 publications.

      • Durand S  et al. Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. Nat Commun 7:12434 (2016). WB . PubMed: 27511142
      • Melero R  et al. The RNA helicase DHX34 functions as a scaffold for SMG1-mediated UPF1 phosphorylation. Nat Commun 7:10585 (2016). IP . PubMed: 26841701
      • Meikar O  et al. An atlas of chromatoid body components. RNA 20:483-95 (2014). ICC/IF ; Mouse . PubMed: 24554440
      • Hogg JR & Goff SP Upf1 senses 3'UTR length to potentiate mRNA decay. Cell 143:379-89 (2010). WB ; Human . PubMed: 21029861

      Images

      • Western blot - Anti-Smg1 antibody (ab30916)
        Western blot - Anti-Smg1 antibody (ab30916)
        All lanes : Anti-Smg1 antibody (ab30916) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
        Lane 2 : Jurkat whole cell lysate (ab7899) at 10 µg
        Lane 3 : A-431 whole cell lysate (ab7909) at 20 µg

        Secondary
        All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

        Performed under reducing conditions.

        Predicted band size: 340 kDa
        Observed band size: 340 kDa



        The band at 340kDa is likely to represent isoform 3 of Smg1. The immunogen is present in all three isoforms of Smg1.   
      • Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916) This image is courtesy of an Abreview submitted by Dr Kirk McManus
        ab30916 (1/200) staining Smg1 by ICC/IF in assynchronous HeLa Cells (green). Cells were countersatined with DAPI (red) in order to show the nucleus. Secondary antibody: Goat anti-Rabbit conjugated to Cy3®. Please refer to abreview for further details.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        Immunocytochemistry/ Immunofluorescence - Anti-Smg1 antibody (ab30916)
        ICC/IF image of ab30916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30916, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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