Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
![Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)](https://www.abcam.com/ps/products/275/ab275963/Images/ab275963-411953-anti-smc3-antibody-immunoprecipitation.jpg "Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR099G] to SMC3 - BSA free
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SMC3 antibody [BLR099G] - BSA free
See all SMC3 primary antibodies -
Description
Rabbit monoclonal [BLR099G] to SMC3 - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human SMC3 aa 1175-1217 (C terminal). The exact sequence is proprietary.
Database link: Q9UQE7 -
Positive control
- WB: HEK-293T, HeLa, Jurkat, LNCaP, A549, MCF-7, GaMG, C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, CH27 cells IP: 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99.01% Borate buffered saline -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
BLR099G -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. ab275963 used for IP at 6 µl per reaction (Lane 1). For blotting immunoprecipitated SMC3, ab275963 was used at 1/1000 dilution. Lane 3 shows a control IgG.
Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)](https://www.abcam.com/ps/products/275/ab275963/Images/ab275963-411951-AntiSMC3-antibody-BLR099G-BSA-free-westernblot-C2C12-70Z-3-BW51473-CTLL2-EL4-RenCa-NIH-3T3-TCMK1-A20-Ba-F3-CH27-cells.JPG)
Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)All lanes : Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution
Lane 1 : C2C12 whole cell lysate
Lane 2 : 70Z/3 whole cell lysate
Lane 3 : BW5147.3 whole cell lysate
Lane 4 : CTLL-2 whole cell lysate
Lane 5 : RenCa whole cell lysate
Lane 6 : NIH/3T3 whole cell lysate
Lane 7 : TCMK-1 whole cell lysate
Lane 8 : A20 whole cell lysate
Lane 9 : Ba/F3 whole cell lysate
Lane 10 : CH27 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 141 kDaChemiluminescence with an exposure time of 10 seconds.
Lower Panel: Rabbit anti-COPB2 antibody
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![Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)](https://www.abcam.com/ps/products/275/ab275963/Images/ab275963-411950-AntiSMC3-antibody-BLR099G-BSA-free-westernblot-HEK293T-HeLa-Jurkat-LNCaP-A549-MCF7-and-GaMG-cells.JPG)
Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)All lanes : Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LNCaP whole cell lysate
Lane 5 : A549 whole cell lysate
Lane 6 : MCF-7 whole cell lysate
Lane 7 : GaMG whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 141 kDaChemiluminescence with an exposure time of 10 seconds.
Lower Panel: Rabbit antiCOPB2 antibody
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![Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)](https://www.abcam.com/ps/products/275/ab275963/Images/ab275963-4-benefits-of-recombinant-antibodies.png)
Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
