Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR099G] to SMC3 - BSA free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SMC3 antibody [BLR099G] - BSA free
    See all SMC3 primary antibodies

  • Description

    Rabbit monoclonal [BLR099G] to SMC3 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SMC3 aa 1175-1217 (C terminal). The exact sequence is proprietary.
    Database link: Q9UQE7

  • Positive control

    • WB: HEK-293T, HeLa, Jurkat, LNCaP, A549, MCF-7, GaMG, C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, CH27 cells IP: 293T cells.

Properties

Images

  • Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    Immunoprecipitation - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)

    Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. ab275963 used for IP at 6 µl per reaction (Lane 1). For blotting immunoprecipitated SMC3, ab275963 was used at 1/1000 dilution. Lane 3 shows a control IgG.

    Chemiluminescence with an exposure time of 10 seconds.

  • Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    All lanes : Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution

    Lane 1 : C2C12 whole cell lysate
    Lane 2 : 70Z/3 whole cell lysate
    Lane 3 : BW5147.3 whole cell lysate
    Lane 4 : CTLL-2 whole cell lysate
    Lane 5 : RenCa whole cell lysate
    Lane 6 : NIH/3T3 whole cell lysate
    Lane 7 : TCMK-1 whole cell lysate
    Lane 8 : A20 whole cell lysate
    Lane 9 : Ba/F3 whole cell lysate
    Lane 10 : CH27 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 141 kDa



    Chemiluminescence with an exposure time of 10 seconds.

    Lower Panel: Rabbit anti-COPB2 antibody

  • Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    Western blot - Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    All lanes : Anti-SMC3 antibody [BLR099G] - BSA free (ab275963) at 1/1000 dilution

    Lane 1 : HEK-293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : LNCaP whole cell lysate
    Lane 5 : A549 whole cell lysate
    Lane 6 : MCF-7 whole cell lysate
    Lane 7 : GaMG whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 141 kDa



    Chemiluminescence with an exposure time of 10 seconds.

    Lower Panel: Rabbit antiCOPB2 antibody

  • Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)
    Anti-SMC3 antibody [BLR099G] - BSA free (ab275963)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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