Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250) - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free
    See all Smad2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2855(2)] to Smad2 (phospho S250) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cell lysate treated with alkaline phosphatase; HeLa cells.

  • General notes

    Ab230098 is the carrier-free version of ab184557. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230098 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)
    Western blot - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)
    All lanes : Anti-Smad2 (phospho S250) antibody [EPR2855(2)] (ab184557) at 1/1000 dilution

    Lane 1 : untreated HeLa cells lysates
    Lane 2 : HeLa cell lysate treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab184557, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)
    Flow Cytometry - Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)
    This data was developed using ab184557, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed and permeabilized HeLa cells labeling Smad2 (phospho S250) with ab184557 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. HeLa cells labeled using ab184557 preincubated with Smad2 (phospho S250) peptide (blue) or non-phospho-Smad2 peptide (orange).
  • Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)
    Anti-Smad2 (phospho S250) antibody [EPR2855(2)] - BSA and Azide free (ab230098)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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