Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)
![Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)](https://www.abcam.com/ps/products/75/ab75273/Images/ab75273-90380-anti-smad1smad5-antibody-af10b7-western-blot.jpg "Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)")
Key features and details
- Mouse monoclonal [AF10B7] to SMAD1+SMAD5
- Suitable for: ELISA, ChIP, Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-SMAD1+SMAD5 antibody [AF10B7] -
Description
Mouse monoclonal [AF10B7] to SMAD1+SMAD5 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, ChIP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant human protein purified from E.coli (His-Smad1)
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Positive control
- SK-N-MC, Ramos, 293T and PC12 cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR188031 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES, 0.01% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
AF10B7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)All lanes : Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273) at 1/1000 dilution
Lane 1 : SK-N-MC cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC12 cell lysate
Predicted band size: 52 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.
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![Flow Cytometry - Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)](https://www.abcam.com/ps/products/75/ab75273/Images/ab75273-90379-anti-smad1smad5-antibody-af10b7-flow-cytometry.jpg)
Flow Cytometry - Anti-SMAD1+SMAD5 antibody [AF10B7] (ab75273)Overlay histogram showing HeLa cells stained with ab75273 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75273, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
