Anti-Smad1 antibody [913C1b] (ab53745)
![Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.com/ps/products/53/ab53745/Images/ab53745-66598-anti-smad1-antibody-913c1b-flow-cytometry.jpg "Anti-Smad1 antibody [913C1b] (ab53745)")
Key features and details
- Mouse monoclonal [913C1b] to Smad1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Smad1 antibody [913C1b]
See all Smad1 primary antibodies -
Description
Mouse monoclonal [913C1b] to Smad1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant Smad1
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Positive control
- WB: HEK293, F2408 and NIH3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
913C1b -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Smad1 antibody [913C1b] (ab53745)Using 0.5µg Anti-SMAD1 antibody (ab53745) and 0.25µg Goat anti-Mouse secondary.
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![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.com/ps/products/53/ab53745/Images/ab53745-66597-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.com/ps/products/53/ab53745/Images/ab53745-66596-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.com/ps/products/53/ab53745/Images/ab53745-66595-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
