Anti-Smad3 (phospho S204) antibody (ab63402)
Key features and details
- Rabbit polyclonal to Smad3 (phospho S204)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Smad3 (phospho S204) antibody
See all Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad3 (phospho S204) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Smad3 only when phosphorylated at serine 204. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
A synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 (AGSPPN).
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Positive control
- Extracts from NIH/3T3 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Smad3 (phospho S204) antibody (ab63402)All lanes : Anti-Smad3 (phospho S204) antibody (ab63402) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells,
treated with Serum (20%, 15mins)
Lane 2 : Extracts from NIH/3T3 cells,
treated with Serum (20%, 15mins) plus phospho peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.