Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6630] to SLC22A3/OCT3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC22A3/OCT3 antibody [EPR6630]
    See all SLC22A3/OCT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR6630] to SLC22A3/OCT3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SLC22A3/OCT3 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as SLC22A3 / EMT

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    All lanes : Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human muscle lysate
    Lane 3 : Human placenta lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Flow Cytometry - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)

    ab124826, at 1/100 dilution, staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
    Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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