Anti-SLC12A3 antibody (ab233401)
Key features and details
- Rabbit polyclonal to SLC12A3
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-SLC12A3 antibody
See all SLC12A3 primary antibodies -
Description
Rabbit polyclonal to SLC12A3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-tag) corresponding to Human SLC12A3 aa 1-150. N-terminal tag. Expressed in E.coli
Database link: P55017 -
Positive control
- IHC-P: Human breast, kidney, colorectal cancer, glioma, liver and stomach tissues. WB: Recombinant human SLC12A3 protein; Pig kidney lysate; HEK-293T cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SLC12A3 antibody (ab233401)All lanes : Anti-SLC12A3 antibody (ab233401) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig kidney tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human stomach tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human liver tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human glioma tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human kidney tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC12A3 antibody (ab233401)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SLC12A3 using ab233401 at 10 µg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
-
Western blot - Anti-SLC12A3 antibody (ab233401)Anti-SLC12A3 antibody (ab233401) at 2 µg/ml + Recombinant human SLC12A3 protein
