Anti-SKI antibody (ab19864)
Key features and details
- Rabbit polyclonal to SKI
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SKI antibody
See all SKI primary antibodies -
Description
Rabbit polyclonal to SKI -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Dog, Xenopus laevis
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human c-Ski.
Read Abcam's proprietary immunogen policy (Peptide available as ab20438.) -
Positive control
- This antibody gave a positive signal in Hela Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab19864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa). Target
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Function
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. -
Sequence similarities
Belongs to the SKI family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6497 Human
- Entrez Gene: 20481 Mouse
- Entrez Gene: 394271 Xenopus laevis
- Omim: 164780 Human
- SwissProt: P49140 Chicken
- SwissProt: P12755 Human
- SwissProt: Q60698 Mouse
- SwissProt: Q02225 Xenopus laevis
see all -
Alternative names
- Avian sarcoma viral (v ski) oncogene homolog antibody
- C oncogene antibody
- C ski antibody
see all
Images
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Western blot - Anti-SKI antibody (ab19864)Anti-SKI antibody (ab19864) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-SKI antibody (ab19864)ICC/IF image of ab19864 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab19864 has been referenced in 3 publications.
- Taguchi L et al. c-Ski accelerates renal cancer progression by attenuating transforming growth factor ß signaling. Cancer Sci 110:2063-2074 (2019). PubMed: 30972853
- Hatley ME et al. Modulation of K-Ras-Dependent Lung Tumorigenesis by MicroRNA-21. Cancer Cell 18:282-93 (2010). WB ; Mouse . PubMed: 20832755
- O TM et al. Differential expression of SKI oncogene protein in hemangiomas. Otolaryngol Head Neck Surg 141:213-8 (2009). PubMed: 19643254
Images
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Western blot - Anti-SKI antibody (ab19864)Anti-SKI antibody (ab19864) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-SKI antibody (ab19864)ICC/IF image of ab19864 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
