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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-SHP1 (phospho Y564) antibody (ab192669)

Anti-SHP1 (phospho Y564) antibody (ab192669)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SHP1 (phospho Y564)
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHP1 (phospho Y564) antibody
    See all SHP1 primary antibodies
  • Description

    Rabbit polyclonal to SHP1 (phospho Y564)
  • Host species

    Rabbit
  • Specificity

    ab192669 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 564.
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
  • Immunogen

    Synthetic peptide within Human SHP1 (phospho Y564) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (D-V-Y(p)-E-N).
    Database link: P29350

  • Positive control

    • WB: JK cells; ICC: HeLa cells; IHC: Mouse liver tissue.
  • General notes

    Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192669 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y564) antibody (ab192669)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 (phospho Y564) antibody (ab192669)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using ab192669

  • Immunocytochemistry - Anti-SHP1 (phospho Y564) antibody (ab192669)
    Immunocytochemistry - Anti-SHP1 (phospho Y564) antibody (ab192669)

    Immunofluorescence staining of methanol-fixed Hela cells using ab192669

  • Western blot - Anti-SHP1 (phospho Y564) antibody (ab192669)
    Western blot - Anti-SHP1 (phospho Y564) antibody (ab192669)

    Western blot analysis of extracts from JK cells, using ab192669.

    Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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