Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-SFRS9/SRSF9 antibody [OTI5G7]
See all SFRS9/SRSF9 primary antibodies
Description

Mouse monoclonal [OTI5G7] to SFRS9/SRSF9
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human SFRS9/SRSF9 aa 1 to the C-terminus. Produced in E.coli (NP_003760).
Database link: Q13242

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Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SFRS9/SRSF9 cDNA. IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, kidney, kidney carcinoma, lung carcinoma, pancreas, pancreas carcinoma, thyroid carcinoma, prostate, prostate carcinoma and lymph node tissues.
General notes

Clone OTI5G7 (formerly 5G7).
This product was previously labelled as SFRS9

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI5G7
Isotype

IgG2b
Research areas

Western blot - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

All lanes : Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414) at 1/2000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SFRS9/SRSF9 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 26 kDa
Western blot - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Lanes 1-2 : Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414) at 1/500 dilution
Lane 3 : Anti-HSP90AB1
Lane 4 : Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414) at 1/500 dilution (Anti-HSP90AB1)

Lane 1 : Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lanes 2 & 4 : SFRS9/SRSF9 knockout HEK-293T whole cell lysate
Lane 3 : Wild-type HEK-293T whole cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human breast adenocarcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human colon adenocarcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human kidney tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human kidney carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human lung carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human pancreas tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human pancreas carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human thyroid carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human prostate tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human prostate carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414)

Paraffin-embedded human lymph node tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.