Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)
![Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)](https://www.abcam.com/ps/products/251/ab251043/Images/ab251043-402931-anti-serum-amyloid-a-antibody-epr11592-129-bsa-and-azide-free-western-blot.jpg "Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11592-129] to Serum Amyloid A - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free
See all Serum Amyloid A primary antibodies -
Description
Rabbit monoclonal [EPR11592-129] to Serum Amyloid A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251043 is the carrier-free version of ab190801. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251043 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR11592-129 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)All lanes : Anti-Serum Amyloid A antibody [EPR11592-129] (ab190801) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?This data was developed using ab190801, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)](https://www.abcam.com/ps/products/251/ab251043/Images/ab251043-399048-anti-serum-amyloid-a-antibody-epr11592-129-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)Anti-Serum Amyloid A antibody [EPR11592-129] (ab190801) at 1/5000 dilution + Human Serum Amyloid A2 recombinant protein (UniProt P0DJI9) with proprietary tag at 0.01 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?This data was developed using ab190801, the same antibody clone in a different buffer formulation.
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![Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)](https://www.abcam.com/ps/products/251/ab251043/Images/ab251043-3-benefits-of-recombinant-antibodies.png)
Anti-Serum Amyloid A antibody [EPR11592-129] - BSA and Azide free (ab251043)
