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Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free
    See all SERPINB1/PI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HeLa cells; Flow Cyt: HeLa cells. WB: HeLa and human lung cells.

  • General notes

    ab250334 is the carrier-free version of ab181084 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SERPINB1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    All lanes : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/2000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Human lung lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 43 kDa



    This data was developed using ab181084, the same antibody clone in a different buffer formulation.

    We are unsure how to define the extra bands.

  • Flow Cytometry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    Flow Cytometry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    This data was developed using ab181084, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    This data was developed using ab181084, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (8.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)
    Anti-SERPINB1/PI2 antibody [EPR13305(B)] - BSA and Azide free (ab250334)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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