Mouse Plasminogen Total ELISA Kit (PLG) (ab198511)
Key features and details
- Sensitivity: 0.38 ng/ml
- Range: 1 ng/ml - 500 ng/ml
- Sample type: Cell culture media, Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Overview
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Product name
Mouse Plasminogen Total ELISA Kit (PLG)
See all Plasminogen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Known Conc 20 8.4ng/ml 0.575 6.85% Known Conc 20 13.2ng/ml 0.884 6.72% Known Conc 20 150ng/ml 11.4 7.61% Inter-assay Sample n Mean SD CV% Known Conc 10 7.93ng/ml 0.736 9.29% Known Conc 10 14ng/ml 1.33 9.51% Known Conc 10 149ng/ml 8.76 5.87% -
Sample type
Urine, Serum, Plasma, Tissue Extracts, Cell culture media -
Assay type
Quantitative -
Sensitivity
0.38 ng/ml -
Range
1 ng/ml - 500 ng/ml -
Recovery
99.25 %
Sample specific recovery Sample type Average % Range Spike = 99.5 96% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's Plasminogen Total (PLG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total plasminogen in mouse plasma, serum, urine, cell culture media, or tissue extracts.
Mouse plasminogen will bind to the capture antibody coated on the microtiter plate. Plasminogen, plasmin, and plasmin in complex with antiplasmin will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-mouse plasminogen primary antibody binds to the plasminogen. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of total plasminogen in the sample.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-mouse plasminogen primary antibody (lyophilized) 1 vial Anti-rabbit horseradish peroxidase secondary antibody 1 vial Mouse plasminogen standard (lyophilized) 1 vial PLG antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests TMB Substrate Solution 1 x 10ml -
Research areas
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Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
Tissue specificity
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
Involvement in disease
Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Domain
Kringle domains mediate interaction with CSPG4. -
Post-translational
modificationsN-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
Cellular localization
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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Alternative names
- Plasmin
- Plasmin heavy chain A
- Plasmin light chain B
see all -
Database links
- Entrez Gene: 18815 Mouse
- SwissProt: P20918 Mouse
- Unigene: 971 Mouse
Images
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Standard Curve.
Full range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.
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Standard Curve.Linear range standard curve using ab198511 Plasminogen Total (PLG) Mouse ELISA Kit.