Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23530-3] to Serotonin transporter - BSA and Azide free
  • Suitable for: IHC-Fr, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free
    See all Serotonin transporter primary antibodies

  • Description

    Rabbit monoclonal [EPR23530-3] to Serotonin transporter - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat cerebrum tissue. IHC-Fr: Mouse cerebrum tissue. IP: Mouse and rat brain tissue lysate.

  • General notes

    ab275094 is the carrier-free version of ab254358.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on synapses of neurons in mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Serotonin transporter was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab254358 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg

    Lane 2: ab254358 IP in mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254358 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets and negative staining on hepatocytes of mouse liver (PMID: 7869097). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Serotonin transporter with ab254358 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID 23127756). The section was incubated with ab254358 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunohistochemistry (Frozen sections) - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Serotonin transporter with ab254358 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on mouse liver (PMID: 9037532) is observed. 

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Immunoprecipitation - Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

    This data was developed using ab254358, the same antibody clone in a different buffer formulation.

    Serotonin transporter was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab254358 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254358 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 µg

    Lane 2: ab254358 IP in rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254358 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 33 seconds.

     

  • Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)
    Anti-Serotonin transporter antibody [EPR23530-3] - BSA and Azide free (ab275094)

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