Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serine racemase antibody [EPR16682] (ab200833)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16682] to Serine racemase
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Serine racemase antibody [EPR16682]
    See all Serine racemase primary antibodies

  • Description

    Rabbit monoclonal [EPR16682] to Serine racemase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LNCaP, U-87 MG and HEK293 whole cell lysates; Human fetal brain lysate. IP: HEK293 and LNCaP whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833)
    Western blot - Anti-Serine racemase antibody [EPR16682] (ab200833)
    All lanes : Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilution

    Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : HEK293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    Blocking/Dilution Buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)
    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

  • Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)
    Immunoprecipitation - Anti-Serine racemase antibody [EPR16682] (ab200833)

    Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: LNCaP whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

  • Anti-Serine racemase antibody [EPR16682] (ab200833)
    Anti-Serine racemase antibody [EPR16682] (ab200833)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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