Anti-Serglycin antibody (ab156991)
Key features and details
- Rabbit polyclonal to Serglycin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Serglycin antibody -
Description
Rabbit polyclonal to Serglycin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: White Adipose Tissue from Subcutaneous & Visceral Fat - Fasted; White Adipose Tissue from Gonad - High Fat Diet (Male); White Adipose Tissue from Gonad - High Fat Diet (Female).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Target
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Function
Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization. -
Sequence similarities
Belongs to the serglycin family. -
Post-translational
modificationsO-glcosylated; contains chondroitin sulfate and heparan sulfate. -
Cellular localization
Cytoplasmic granule. Secreted > extracellular space. Golgi apparatus. Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation. Secreted constitutively by endothelial cells and macrophages. Located to Golgi apparatus during neutrophil differentiation. - Information by UniProt
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Database links
- Entrez Gene: 19073 Mouse
- Entrez Gene: 56782 Rat
- SwissProt: P13609 Mouse
- SwissProt: P04917 Rat
- Unigene: 338790 Mouse
- Unigene: 40122 Rat
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Alternative names
- Chondroitin sulfate proteoglycan core protein antibody
- Cytolytic granule proteoglycan core protein antibody
- FLJ12930 antibody
see all
Images
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Western blot - Anti-Serglycin antibody (ab156991)All lanes : Anti-Serglycin antibody (ab156991) at 1 µg/ml
Lane 1 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate
Lane 2 : Mouse (Male) WAT from Gonad (High Fat Diet) Tissue Lysate
Lane 3 : Mouse (Female) WAT from Gonad (High Fat Diet) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 14 kDa (possible non-specific binding), 30 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156991 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab156991 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Serglycin antibody (ab156991)All lanes : Anti-Serglycin antibody (ab156991) at 1 µg/ml
Lane 1 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate
Lane 2 : Mouse (Male) WAT from Gonad (High Fat Diet) Tissue Lysate
Lane 3 : Mouse (Female) WAT from Gonad (High Fat Diet) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 14 kDa (possible non-specific binding), 30 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156991 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.