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Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)

Price and availability

526 012 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16099] to Septin 8 - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Septin 8 antibody [EPR16099] - BSA and Azide free
    See all Septin 8 primary antibodies
  • Description

    Rabbit monoclonal [EPR16099] to Septin 8 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251065 is the carrier-free version of ab191404. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251065 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16099
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis

Images

  • Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This data was developed using ab191404, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : U-87 MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This data was developed using ab191404, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Western blot - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    All lanes : Anti-Septin 8 antibody [EPR16099] (ab191404) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa



    This data was developed using ab191404, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    This data was developed using ab191404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human stomach tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    This data was developed using ab191404, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling Septin 8 with ab191404 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Immunocytochemistry - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    This data was developed using ab191404, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized C6 cells labeling Septin 8 with ab191404 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).The two negative controls used anti-Septin 8 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
  • Flow Cytometry - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Flow Cytometry - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    This data was developed using ab191404, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling Septin 8 with ab191404 at 1/150 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti-rabbit IgG (FITC) was used as secondary antibody at 1/150 dilution.
  • Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Immunoprecipitation - Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)

    This data was developed using ab191404, the same antibody clone in a different buffer formulation.

    Western blot analysis of Septin 8 in K562 cell lysate immunoprecipitated using ab191404 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)
    Anti-Septin 8 antibody [EPR16099] - BSA and Azide free (ab251065)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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