Anti-CHD6 antibody [EPR23857-29] (ab259858)
![Anti-CHD6 antibody [EPR23857-29] (ab259858)](https://www.abcam.com/ps/products/259/ab259858/Images/ab259858-459580-anti-chd6-antibody-epr2385729-western-blot-adenocarcinoma.jpg "Anti-CHD6 antibody [EPR23857-29] (ab259858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23857-29] to CHD6
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CHD6 antibody [EPR23857-29]
See all CHD6 primary antibodies -
Description
Rabbit monoclonal [EPR23857-29] to CHD6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23857-29 -
Isotype
IgG
Images
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Western blot - Anti-CHD6 antibody [EPR23857-29] (ab259858)All lanes : Anti-CHD6 antibody [EPR23857-29] (ab259858) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 305 kDa
Observed band size: 315 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: 158 seconds
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(ab259858)