Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13270(B)] to SEC23A - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free
    See all SEC23A primary antibodies

  • Description

    Rabbit monoclonal [EPR13270(B)] to SEC23A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250116 is the carrier-free version of ab179811 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13270(B)

  • Isotype

    IgG

Images

  • Western blot - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    Western blot - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)

    This data was developed using ab179811, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: SEC23A knockout HAP1 cell lysate (20 µg) 

    Lane 3: HepG2 cell lysate (20 µg)

    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab179811 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab179811 was shown to specifically react with SEC23A when SEC23A knockout samples were used. Wild-type and SEC23A knockout samples were subjected to SDS-PAGE. ab179811 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    Western blot - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    All lanes : Anti-SEC23A antibody [EPR13270(B)] (ab179811) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab179811, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    Flow Cytometry - Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    This data was developed using ab179811, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized HeLa cells labeling SEC23A with ab179811 at 1/10 (red) or a rabbit IgG (negative) (green).
  • Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)
    Anti-SEC23A antibody [EPR13270(B)] - BSA and Azide free (ab250116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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