Anti-SCN4B antibody (ab80539)
Key features and details
- Rabbit polyclonal to SCN4B
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-SCN4B antibody
See all SCN4B primary antibodies -
Description
Rabbit polyclonal to SCN4BThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
-
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB RatHuman
-
Immunogen
Synthetic peptide corresponding to Human SCN4B aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab95413) -
Positive control
- Brain (Human) Tissue Lysate; Brain (Rat) Tissue Lysate ; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate ; Spinal Cord (Human) Tissue Lysate; Spinal Cord (Rat) Tissue Lysate ; Skeletal Muscle (Human) Tissue Lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF RatWB RatHumanAll applications MouseCowApplication Abreviews Notes WB (2) Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.ICC/IF Use at an assay dependent concentration.Notes WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.ICC/IF
Use at an assay dependent concentration.Target
-
Relevance
The SCN4B protein modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation By similarity. The protein has been found to be expressed at a high level in dorsal root ganglia, at a lower level in brain, spinal cord, skeletal muscle and heart. Defects in SCN4B are the cause of long QT syndrome type 10 (LQT10) [MIM:611819]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 6330 Human
- SwissProt: Q8IWT1 Human
-
Alternative names
- SCN4B_HUMAN antibody
- Sodium channel subunit beta-4 antibody
Images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.-
Western blot - Anti-SCN4B antibody (ab80539)All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 4 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 5 : Spinal Cord (Rat) Tissue Lysate
Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. SCN4B contains at least three potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted. The primary sequence contains a 30-residue signal sequence at the N-terminus. The band around 20-kDa in human skeletal muscle may be the unglycosylated form of the target protein. -
Immunocytochemistry/ Immunofluorescence - Anti-SCN4B antibody (ab80539)ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (6)
ab80539 has been referenced in 6 publications.
- Sheilabi MA et al. The Sodium Channel B4-Subunits are Dysregulated in Temporal Lobe Epilepsy Drug-Resistant Patients. Int J Mol Sci 21:N/A (2020). PubMed: 32331418
- Xiao Y et al. Increased Resurgent Sodium Currents in Nav1.8 Contribute to Nociceptive Sensory Neuron Hyperexcitability Associated with Peripheral Neuropathies. J Neurosci 39:1539-1550 (2019). PubMed: 30617209
- Novati A et al. Environment-dependent striatal gene expression in the BACHD rat model for Huntington disease. Sci Rep 8:5803 (2018). PubMed: 29643462
- Xie W et al. Upregulation of the sodium channel NaVß4 subunit and its contributions to mechanical hypersensitivity and neuronal hyperexcitability in a rat model of radicular pain induced by local dorsal root ganglion inflammation. Pain 157:879-91 (2016). PubMed: 26785322
- Barbosa C et al. Navß4 regulates fast resurgent sodium currents and excitability in sensory neurons. Mol Pain 11:60 (2015). PubMed: 26408173
- Tan ZY et al. Tetrodotoxin-resistant sodium channels in sensory neurons generate slow resurgent currents that are enhanced by inflammatory mediators. J Neurosci 34:7190-7 (2014). PubMed: 24849353
Images
-
Western blot - Anti-SCN4B antibody (ab80539)All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 4 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 5 : Spinal Cord (Rat) Tissue Lysate
Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. SCN4B contains at least three potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted. The primary sequence contains a 30-residue signal sequence at the N-terminus. The band around 20-kDa in human skeletal muscle may be the unglycosylated form of the target protein. -
Immunocytochemistry/ Immunofluorescence - Anti-SCN4B antibody (ab80539)ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
