Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Scc4 antibody [EPR14390] (ab183033)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14390] to Scc4
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Scc4 antibody [EPR14390]
    See all Scc4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14390] to Scc4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT1080, HeLa, MCF7 and 293 cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14390

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Scc4 antibody [EPR14390] (ab183033)
    Western blot - Anti-Scc4 antibody [EPR14390] (ab183033)
    All lanes : Anti-Scc4 antibody [EPR14390] (ab183033) at 1/5000 dilution

    Lane 1 : HT1080 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 69 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033)
    Immunocytochemistry/ Immunofluorescence - Anti-Scc4 antibody [EPR14390] (ab183033)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling Scc4 with ab183033 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)
    Immunoprecipitation - Anti-Scc4 antibody [EPR14390] (ab183033)

    Western blot analysis of immunoprecipitation pellet from Human 293 lysate immunoprecipitated using ab183033 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Flow Cytometry - Anti-Scc4 antibody [EPR14390] (ab183033)
    Flow Cytometry - Anti-Scc4 antibody [EPR14390] (ab183033)

    Flow Cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%)  labeling Scc4 with ab183033 (red) at 1/90 dilution or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-Scc4 antibody [EPR14390] (ab183033)
    Anti-Scc4 antibody [EPR14390] (ab183033)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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