Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4128] to SCAI - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-SCAI antibody [EPR4128] - BSA and Azide free
See all SCAI primary antibodies -
Description
Rabbit monoclonal [EPR4128] to SCAI - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab247960 is the carrier-free version of ab124688. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247960 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 5.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4128 -
Isotype
IgG -
Research areas
Images
-
This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining. -
All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. -
This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control. -
All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)
Lane 1 : Human Thymus lysate
Lane 2 : Human Thyroid lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDaThis data was developed using ab124688, the same antibody clone in a different buffer formulation.
-
All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?This data was developed using ab124688, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab124688, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
-
This data was developed using ab124688, the same antibody clone in a different buffer formulation.Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
-
This data was developed using ab124688, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
-