Anti-SOS2 antibody (ab85831)
Key features and details
- Rabbit polyclonal to SOS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOS2 antibody
See all SOS2 primary antibodies -
Description
Rabbit polyclonal to SOS2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1282 -1332 of Human SOS2 (NP_008870.2)
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Positive control
- HeLa and 293T whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOS2 antibody (ab85831)All lanes : Anti-SOS2 antibody (ab85831) at 0.1 mg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 153 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-SOS2 antibody (ab85831)Immunoprecipitation/ Western Blot of ab85831
Lane 1: ab85831 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab85831 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
