Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843)
Key features and details
- Rabbit polyclonal to Sca1 / Ly6A/E - Hematopoietic Stem Cell Marker
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker
See all Sca1 / Ly6A/E primary antibodies -
Description
Rabbit polyclonal to Sca1 / Ly6A/E - Hematopoietic Stem Cell Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Sca1/ Ly6A/E aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab104951) -
Positive control
- This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).Target
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Function
T-cell activation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsO-glycosylated. Not N-glycosylated.
Not phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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Alternative names
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
Images
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Western blot - Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843)All lanes : Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.
Datasheets and documents
References (0)
ab93843 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843)All lanes : Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.
