Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µg 351 792 ₸ 10 µg 117 264 ₸

Order now and get it on Wednesday March 10, 2021

Anti-SATB1 antibody [14/SATB1] (ab280578)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [14/SATB1] to SATB1
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-SATB1 antibody [14/SATB1]
See all SATB1 primary antibodies
Description

Mouse monoclonal [14/SATB1] to SATB1
Host species

Mouse
Tested Applications & Species

Application Species
WB
Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat thymus tissue lysate; Jurkat, THP1 cell lysates. IHC-P: Human thymus, tonsil; Mouse thymus; Rat thymus.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

14/SATB1
Isotype

IgG1
Research areas

Western blot - Anti-SATB1 antibody [14/SATB1] (ab280578)

All lanes : Anti-SATB1 antibody [14/SATB1] (ab280578) at 1/1000 dilution

Lane 1 : Rat thymus tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : THP1 (human monocytic leukemia monocyte), whole cell lysate
Lane 5 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 85 kDa

Blocking buffer and Diluting buffer concentration: 5% NFDM/TBST

Negative control: liver tissue (PMID: 1505028).

Exposure time: 15 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human thymus. The section was incubated with ab280578 for 30 minutes at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil. The section was incubated with ab280578 for 30 minutes at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 minutes.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse thymus. The section was incubated with ab280578 for 30 minutes at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat thymus. The section was incubated with ab280578 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] (ab280578)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Anti-SATB1 antibody [14/SATB1] (ab280578)

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