Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike RBD stained with ab281304 at 1/600 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with ab281304 (160-1.91*10^-5 nM). HRP-Rabbit IgG antibody at 1/10000 dilituion detected bound primary antibody.
EC50 : 25.13 pM

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at 5 µg/mL and ab281304 as detection antibody at concentration of 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution and used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) coated at 2 µg/mL binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag at 3000 ng/mL. ACE2 binding was inhibited by increasing concentrations of ab281304 (14-10000 ng/mL). Bound ACE2 protein was detected by HRP-Goat Anti-Mouse IgG antibody at 1/10000 dilution.

Sandwich ELISA detection of non-transfected and SARS-CoV-2 (COVID-19) Spike, MERS-CoV Spike, or SARS-CoV Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.
Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (4000-62.5 ng/mL). Coated protein was probed with ab281304 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution was used to detect bound primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugatedab281304 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281304 needs to be conjugated to HRP to function as the detection antibody.