Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)
![Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-456634-SARSCoV2-nucleocapsid-protein-antibody-HL344-wb.jpg "Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL344] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: WB, ICC, IHC-P, Sandwich ELISA
Overview
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Product name
Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free
See all SARS-CoV-2 nucleocapsid protein primary antibodies -
Description
Rabbit monoclonal [HL344] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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Positive control
- IHC-P: SARS-CoV-2 (COVID-19) infected human placenta and lung tissue, SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL344 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281296)Paraffin embedded SARS-CoV-2 (COVID-19) infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281296 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281296)Paraffin embedded SARS-CoV-2 (COVID-19) infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281296 in immunohistochemical analysis.
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-458185-SARSCoV2-nucleocapsid-antibody-HL344-elisa.JPG)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (A and B) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with ab281296 at 1 μg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-6-SARSCoV2-COVID19-nucleocapsid-antibody-HL344-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281296 as capture antibody at concentration of 5 µg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281296)Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281296 (green) at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate bufferat pH 6.0 for 15 minutes.
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![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-5-SARSCoV2-COVID19-nucleocapsid-antibody-HL344-immunocytochemistry-293t-cells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)Mock and transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281296 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-458187-SARSCoV2-nucleocapsid-antibody-HL344-elisa2.JPG)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281296 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.074 nM -
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)](https://www.abcam.com/ps/products/281/ab281296/Images/ab281296-2-SARSCoV2-COVID19-nucleocapsid-antibody-HL344-westernblot-293tcells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL344] - BSA and Azide free (ab281296) at 1/5000 dilution
Lane 1 : 293T whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
