Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sall4 antibody [SP289] (ab226756)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP289] to Sall4
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Sall4 antibody [SP289]
    See all Sall4 primary antibodies

  • Description

    Rabbit monoclonal [SP289] to Sall4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human seminoma, testis, and Human ovarian carcinoma tissue; Flow Cyt: NCCIT cells; ICC: NCCIT cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Sall4 with ab226756 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] (ab226756)
    Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] (ab226756)

    Immunocytochemistry/ Immunofluorescence analysis of NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling Sall4 with purified ab226756 at 1/10 (12.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Sall4 antibody [SP289] (ab226756)
    Flow Cytometry - Anti-Sall4 antibody [SP289] (ab226756)

    Flow cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) labeling Sall4 with purified ab226756 at 1/20 dilution (6.15 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

    Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

    Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

    Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

    Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

    Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Sall4 antibody [SP289] (ab226756)
    Anti-Sall4 antibody [SP289] (ab226756)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Sall4 antibody [SP289] (ab226756)

© 2021 Astana Biomed Group LLP. All rights reserved.