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Anti-SALL1 antibody (ab31526)

Key features and details

  • Rabbit polyclonal to SALL1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-SALL1 antibody
    See all SALL1 primary antibodies

  • Description

    Rabbit polyclonal to SALL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Chicken, Human, Drosophila melanogaster

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab31903.)

Properties

Applications

Our Abpromise guarantee covers the use of ab31526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).

Target

  • Function

    Transcriptional repressor involved in organogenesis.

  • Tissue specificity

    Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver.

  • Involvement in disease

    Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
    Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation.

  • Sequence similarities

    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.

  • Developmental stage

    In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • HSal1 antibody
      • Sal like protein 1 antibody
      • Sal-1 antibody
      • Sal-like protein 1 antibody
      • Sal1 antibody
      • SALL1 antibody
      • SALL1_HUMAN antibody
      • Spalt-like transcription factor 1 antibody
      • TBS antibody
      • Zinc finger protein 794 antibody
      • Zinc finger protein SALL1 antibody
      • Zinc finger protein Spalt-1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (ab31526)
      Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (ab31526)

      ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-SALL1 antibody (ab31526)
      Western blot - Anti-SALL1 antibody (ab31526)
      Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 140 kDa
      Observed band size: 200 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744

    References (11)

    Publishing research using ab31526? Please let us know so that we can cite the reference in this datasheet.

    ab31526 has been referenced in 11 publications.

    • Goto T  et al. Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats. Nat Commun 10:451 (2019). PubMed: 30723213
    • Chi D  et al. Spalt-Like Transcription Factor 1 (SALL1) Gene Expression Inhibits Cell Proliferation and Cell Migration of Human Glioma Cells Through the Wnt/ß-Catenin Signaling Pathway. Med Sci Monit Basic Res 25:128-138 (2019). PubMed: 31040265
    • Pluvinage JV  et al. CD22 blockade restores homeostatic microglial phagocytosis in ageing brains. Nature 568:187-192 (2019). PubMed: 30944478
    • Liu H  et al. Histone deacetylases 1 and 2 regulate the transcriptional programs of nephron progenitors and renal vesicles. Development 145:N/A (2018). PubMed: 29712641
    • Reginensi A  et al. A critical role for NF2 and the Hippo pathway in branching morphogenesis. Nat Commun 7:12309 (2016). IF . PubMed: 27480037
    View all Publications for this product

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (ab31526)
      Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (ab31526)

      ICC/IF image of ab31526 stained Mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31526 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-SALL1 antibody (ab31526)
      Western blot - Anti-SALL1 antibody (ab31526)
      Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 140 kDa
      Observed band size: 200 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      Although the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744

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