Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-S100 alpha antibody [EPR5250] (ab133519)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5250] to S100 alpha
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-S100 alpha antibody [EPR5250]
See all S100 alpha primary antibodies
Description

Rabbit monoclonal [EPR5250] to S100 alpha
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in the Human S100 alpha protein (UniProt ID: P23297).
Positive control
- C2C12, Mouse heart and brain cell lysates; Human endometrial carcinoma and heart muscle tissue; Mouse heart tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.80 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5250
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-S100 alpha antibody [EPR5250] (ab133519)

All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution (purified)

Lane 1 : Rat heart lysates at 20 µg
Lane 2 : Human fetal heart lysates at 15 µg

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 10 kDa

Blocking and diluting buffer: 5% NBST/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry analysis of paraffin embedded Human endometrial carcinoma tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-S100 alpha antibody [EPR5250] (ab133519)

Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution + Mouse heart lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 10 kDa
Observed band size: 10 kDa

5%NFDM/TBST
Western blot - Anti-S100 alpha antibody [EPR5250] (ab133519)

All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lane 3 : C2C12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry analysis of paraffin embedded Human heart muscle tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] (ab133519)

Immunohistochemistry analysis of paraffin embedded Mouse heart tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-S100 alpha antibody [EPR5250] (ab133519)

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