Anti-RTT106 antibody (ab130122)
Key features and details
- Rabbit polyclonal to RTT106
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-RTT106 antibody -
Description
Rabbit polyclonal to RTT106 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to Saccharomyces cerevisiae RTT106 aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: P40161 -
Positive control
- This antibody gave a positive signal in both Wild Type Exponentially growing yeast and W303 Saccromyces cerevisiae.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Saccharomyces cerevisiaeApplication Abreviews Notes WB 1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).Notes WB
1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).Target
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Function
Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Involved in regulation of Ty1 transposition. -
Sequence similarities
Belongs to the RTT106 family. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 855515 Saccharomyces cerevisiae
- SwissProt: P40161 Saccharomyces cerevisiae
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Alternative names
- Histone chaperone rtt106 antibody
- Regulator of Ty1 transposition protein 106 antibody
- RT106_YEAST antibody
- rtt106 antibody
Images
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Western blot - Anti-RTT106 antibody (ab130122)All lanes : Anti-RTT106 antibody (ab130122) at 1/250 dilution (Milk blocking 3%)
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab130122 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab130122 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-RTT106 antibody (ab130122)All lanes : Anti-RTT106 antibody (ab130122) at 1/250 dilution (Milk blocking 3%)
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab130122 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
