Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12827] to NASP - BSA and Azide free
- Suitable for: ICC, IP, Flow Cyt (Intra), WB
- Reacts with: Human
Overview
-
Product name
Anti-NASP antibody [EPR12827] - BSA and Azide free
See all NASP primary antibodies -
Description
Rabbit monoclonal [EPR12827] to NASP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250395 is the carrier-free version of ab181169.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12827 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : NTERA2/D1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 85 kDaThis data was developed using ab181169, the same antibody clone in a different buffer formulation.
160KDa: tNASP
62KDa: sNASP.
-
This data was developed using ab181169, the same antibody clone in a different buffer formulation.Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
-