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Epigenetics and Nuclear Signaling Chromatin Remodeling Histone chaperones

Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)

Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12827] to NASP - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt (Intra), WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-NASP antibody [EPR12827] - BSA and Azide free
    See all NASP primary antibodies
  • Description

    Rabbit monoclonal [EPR12827] to NASP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250395 is the carrier-free version of ab181169.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12827
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Histone chaperones
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Images

  • Western blot - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    Western blot - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Caco-2 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : NTERA2/D1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 85 kDa



    This data was developed using ab181169, the same antibody clone in a different buffer formulation.

    160KDa: tNASP

    62KDa: sNASP.

  • Immunoprecipitation - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    Immunoprecipitation - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    This data was developed using ab181169, the same antibody clone in a different buffer formulation.Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
  • Flow Cytometry - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    Flow Cytometry - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    This data was developed using ab181169, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.
  • Immunocytochemistry - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    Immunocytochemistry - Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    This data was developed using ab181169, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
  • Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)
    Anti-NASP antibody [EPR12827] - BSA and Azide free (ab250395)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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