Anti-NASP antibody [EPR12827] (ab181169)
![Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.com/ps/products/181/ab181169/Images/ab181169-214728-anti-nasp-antibody-epr12827-western-blot.jpg "Anti-NASP antibody [EPR12827] (ab181169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12827] to NASP
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
-
Product name
Anti-NASP antibody [EPR12827]
See all NASP primary antibodies -
Description
Rabbit monoclonal [EPR12827] to NASP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, Caco2, 293 or NTERA2/D1 lysate, 293 or HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12827 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NASP antibody [EPR12827] (ab181169)All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : Caco2 lysate
Lane 3 : 293 lysate
Lane 4 : NTERA2/D1 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 85 kDa160KDa: tNASP
62KDa: sNASP -
![Immunoprecipitation - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.com/ps/products/181/ab181169/Images/ab181169-214736-anti-nasp-antibody-epr12827-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NASP antibody [EPR12827] (ab181169)Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
-
![Flow Cytometry - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.com/ps/products/181/ab181169/Images/ab181169-214735-anti-nasp-antibody-epr12827-flow-cytometry.jpg)
Flow Cytometry - Anti-NASP antibody [EPR12827] (ab181169)Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.com/ps/products/181/ab181169/Images/ab181169-214733-anti-nasp-antibody-epr12827-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NASP antibody [EPR12827] (ab181169)Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
-
![Anti-NASP antibody [EPR12827] (ab181169)](https://www.abcam.com/ps/products/181/ab181169/Images/ab181169-5-benefits-of-recombinant-antibodies.png)
Anti-NASP antibody [EPR12827] (ab181169)
