Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7808] to RPS3 - Ribosome Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RPS3 antibody [EPR7808] - Ribosome Marker
See all RPS3 primary antibodies -
Description
Rabbit monoclonal [EPR7808] to RPS3 - Ribosome Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P HumanWB Human -
Immunogen
A synthetic peptide corresponding to residues in Human RPS3 (P23396)
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Positive control
- WB: HepG2, PC-12 and Neuro-2a lysate IHC-P: Human kidney tissue ICC/IF: Neuro-2a cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.36 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7808 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
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Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with ab128995 at 1/100 dilution. ab150077, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.Confocal image showing cytoplasmic staining in Neuro-2a cells.
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ab128995, at a 1/50 dilution, staining RPS3 in Human kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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