Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)
![Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)](https://www.abcam.com/ps/products/247/ab247308/Images/ab247308-405466-anti-hsd17b1-antibody-ep1682y-bsa-and-azide-free-western-blot.jpg "Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1682Y] to HSD17B1 - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free
See all HSD17B1 primary antibodies -
Description
Rabbit monoclonal [EP1682Y] to HSD17B1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Human placenta lysate.
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General notes
ab247308 is the carrier-free version of ab51045 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247308 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1682Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta tissue lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDaThis data was developed using ab51045, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)](https://www.abcam.com/ps/products/247/ab247308/Images/ab247308-2-anti-hsd17b1-antibody-ep1682y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)This data was developed using ab51045, the same antibody clone in a different buffer formulation.Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)](https://www.abcam.com/ps/products/247/ab247308/Images/ab247308-6-anti-hsd17b1-antibody-ep1682y-immunoprecipitation-placenta-human.jpg)
Immunoprecipitation - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)This data was developed using ab51045, the same antibody clone in a different buffer formulation.
Purified ab51045 at 1/50 dilution (2µg) immunoprecipitating HSD17B1 in Human placenta lysate.
Lane 1 (input): Human placenta lysate 10µg
Lane 2 (+): ab51045 + Human placenta lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51045 in Human placenta lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 34 kDa -
![Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)](https://www.abcam.com/ps/products/247/ab247308/Images/ab247308-1-anti-hsd17b1-antibody-ep1682y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)This data was developed using ab51045, the same antibody clone in a different buffer formulation.Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
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![Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)](https://www.abcam.com/ps/products/247/ab247308/Images/ab247308-4-benefits-of-recombinant-antibodies.png)
Anti-HSD17B1 antibody [EP1682Y] - BSA and Azide free (ab247308)