Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16726] to AKR1C3 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free
    See all AKR1C3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16726] to AKR1C3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251511 is the carrier-free version of ab209899. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251511 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Western blot - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Lanes 1-2 : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/1000 dilution
    Lane 3 : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    This data was developed using ab209899, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 3: 2 seconds; Lane 2: 30 seconds.

  • Western blot - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Western blot - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    All lanes : Anti-AKR1C3 antibody [EPR16726] (ab209899) at 1/2000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (control)
    Lanes 2-3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    This data was developed using ab209899, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Immunocytochemistry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    This data was developed using ab209899, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunocytochemistry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Immunocytochemistry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    This data was developed using ab209899, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (Alexa Fluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab209899 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Flow Cytometry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Flow Cytometry - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    This data was developed using ab209899, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling AKR1C3 with ab209899 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Immunoprecipitation - Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    This data was developed using ab209899, the same antibody clone in a different buffer formulation.AKR1C3 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab209899 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209899 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HepG2 whole cell lysate, 10µg (Input). Lane 2: ab209899 IP in HepG2 whole cell lysate. Lane 3: Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab209899 in HepG2 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)
    Anti-AKR1C3 antibody [EPR16726] - BSA and Azide free (ab251511)

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