Anti-RPP40 antibody (ab228728)
Key features and details
- Rabbit polyclonal to RPP40
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RPP40 antibody
See all RPP40 primary antibodies -
Description
Rabbit polyclonal to RPP40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Recombinant fragment within Human RPP40 (internal sequence). The exact sequence is proprietary.
Database link: O75818 -
Positive control
- WB: HEK-293T, HeLa, A431 and HepG2 whole cell extracts. ICC/IF: SK-N-SH cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-RPP40 antibody (ab228728)All lanes : Anti-RPP40 antibody (ab228728) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa10% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-RPP40 antibody (ab228728)SK-N-SH (human neuroblastoma cell line) cells stained for RPP40 (green) using ab228728 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
