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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-RPP25 antibody (ab126338)

Anti-RPP25 antibody (ab126338)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RPP25
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RPP25 antibody
    See all RPP25 primary antibodies
  • Description

    Rabbit polyclonal to RPP25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: ASLSVLKNVP GLAILLSKDA LDPRQPGYQP PNPHPGPSSP PAAPASKRSL GEPAAGEGSA KRSQPEP, corresponding to Human RPP25.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: SK-MEL-30 whole cell lysates; IHC-P: Human colon, kidney and testis tissue; ICC: SiHa whole cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-RPP25 antibody (ab126338)
    Western blot - Anti-RPP25 antibody (ab126338)
    Anti-RPP25 antibody (ab126338) at 0.4 µg/ml + SK-MEL-30 (Human cutaneous melanoma cell line) whole cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)

    Immunohistochemical analysis of human colon tissue labeling RPR25 in the nucleus of glandular cells with ab126338 at a 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (ab126338)
    Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (ab126338)

    Immunocytochemical analysis of SiHa (Human cervical carcinoma cell line) whole cells labeling RPR25 in the nucleoplasm and microtubule organizing center with ab126338 at 2 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)

    Immunohistochemical analysis of human kidney tissue labeling RPR25 in the nucleus of cells in tubules with ab126338 at a 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)

    Immunohistochemical analysis of human testis tissue labeling RPR25 in the nucleus of Leydig cells with ab126338 at a 1/50 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)

    Immunohistochemical analysis of human skeletal muscle tissue labeling RPR25 with ab126338 at a 1/50 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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