Anti-PMM2 antibody (ab229996)
Key features and details
- Rabbit polyclonal to PMM2
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PMM2 antibody
See all PMM2 primary antibodies -
Description
Rabbit polyclonal to PMM2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Human PMM2 aa 1 to the C-terminus.
Database link: O15305 -
Positive control
- WB: A549, HepG2, MCF7, K562, HeLa and HEK-293T whole cell lysates. IHC-P: Human thyroid and gastric cancer tissues. IP: HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PMM2 antibody (ab229996)All lanes : Anti-PMM2 antibody (ab229996) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (ab229996)Paraffin-embedded human thyroid tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (ab229996)Paraffin-embedded human gastric cancer tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-PMM2 antibody (ab229996)PMM2 was immunoprecipitated from 500 µg HeLa (Human breast adenocarcinoma cell line) whole cell lysate using 4 µg of ab229996.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HeLa whole cell lysate.
Lane 2: ab229996 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.
