Anti-RPL3 antibody (ab228638)
Key features and details
- Rabbit polyclonal to RPL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL3 antibody
See all RPL3 primary antibodies -
Description
Rabbit polyclonal to RPL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human RPL3 (internal sequence). The exact sequence is proprietary.
Database link: P39023 -
Positive control
- WB: Jurkat and Raji whole cell lysates. IHC-P: Mouse intestine and brain tissue; 4T1 xenograft tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPL3 antibody (ab228638)All lanes : Anti-RPL3 antibody (ab228638) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 46 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab228638)Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for RPL3 (green) using ab228638 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab228638)Paraffin-embedded mouse intestine tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab228638)Paraffin-embedded 4T1 xenograft tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab228638)Paraffin-embedded mouse brain tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
