Anti-ROCK1 antibody (ab97592)
Key features and details
- Rabbit polyclonal to ROCK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ROCK1 antibody
See all ROCK1 primary antibodies -
Description
Rabbit polyclonal to ROCK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Cow, Zebrafish
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Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 107 and 381 of human ROCK1 (Q13464)
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Positive control
- 293T , A431 , HeLa , HepG2 , NIH-3T3 , C2Cl2 , rat brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ROCK1 antibody (ab97592)All lanes : Anti-ROCK1 antibody (ab97592) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : ROCK1 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 158 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody (ab97592)Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for ROCK1 with ab97592 at 1:500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by an alpha Tubulin antibody diluted at 1:500.
Blue: Hoechst 33342 staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody (ab97592)Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling ROCK1 with ab97592 antibody at 1/500 dilution.
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Western blot - Anti-ROCK1 antibody (ab97592)All lanes :
Lane 1 : 293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 158 kDa5% SDS-PAGE
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Western blot - Anti-ROCK1 antibody (ab97592)Anti-ROCK1 antibody (ab97592) at 1/500 dilution + Rat brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 158 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody (ab97592)ab97592, at a 1/500 dilution, staining human ROCK1 in DLD1 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
