Anti-Robo1 antibody (ab85312)
Key features and details
- Rabbit polyclonal to Robo1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Robo1 antibody
See all Robo1 primary antibodies -
Description
Rabbit polyclonal to Robo1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Robo1 aa 1600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab94835) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. -
Tissue specificity
Widely expressed, with exception of kidney. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsUbiquitinated. May be deubiquitinated by USP33. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6091 Human
- Entrez Gene: 19876 Mouse
- Omim: 602430 Human
- SwissProt: Q9Y6N7 Human
- SwissProt: O89026 Mouse
- Unigene: 13640 Human
- Unigene: 310772 Mouse
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Alternative names
- Deleted in U twenty twenty antibody
- DUTT 1 antibody
- DUTT1 antibody
see all
Images
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Western blot - Anti-Robo1 antibody (ab85312)All lanes : Anti-Robo1 antibody (ab85312) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Human Roundabout homolog 1 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Robo1 antibody (ab85312)ICC/IF image of ab85312 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85312, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References (5)
ab85312 has been referenced in 5 publications.
- Zhan Y et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). PubMed: 31354412
- Smeda M et al. Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res 20:86 (2018). PubMed: 30075800
- Cuesta S et al. Non-Contingent Exposure to Amphetamine in Adolescence Recruits miR-218 to Regulate Dcc Expression in the VTA. Neuropsychopharmacology 43:900-911 (2018). PubMed: 29154364
- Huang YJ et al. Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells. J Cell Sci 130:2459-2467 (2017). PubMed: 28596239
- Shrivastava A et al. Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements. Sci Rep 5:16833 (2015). PubMed: 26582347
Images
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Western blot - Anti-Robo1 antibody (ab85312)All lanes : Anti-Robo1 antibody (ab85312) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Human Roundabout homolog 1 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Robo1 antibody (ab85312)ICC/IF image of ab85312 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85312, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
