Anti-Ribonuclease Inhibitor/RAI antibody (ab217132)
Key features and details
- Rabbit polyclonal to Ribonuclease Inhibitor/RAI
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ribonuclease Inhibitor/RAI antibody
See all Ribonuclease Inhibitor/RAI primary antibodies -
Description
Rabbit polyclonal to Ribonuclease Inhibitor/RAI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human Ribonuclease Inhibitor/RAI aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P13489 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
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General notes
This product was previously labelled as Ribonuclease Inhibitor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab217132 was affinity purified using an epitope specific to Ribonuclease Inhibitor/RAI immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ribonuclease Inhibitor/RAI antibody (ab217132)All lanes : Anti-Ribonuclease Inhibitor/RAI antibody (ab217132) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 10 secondsLysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Ribonuclease Inhibitor/RAI antibody (ab217132)Detection of Ribonuclease Inhibitor/RAI in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab217132 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
