Anti-RHOB antibody (ab155149)
Key features and details
- Rabbit polyclonal to RHOB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RHOB antibody
See all RHOB primary antibodies -
Description
Rabbit polyclonal to RHOB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-146 of Human RHOB (UniProt P62745).
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Positive control
- WB: U-87 MG cell lysate; mouse and rat brain tissue extract. IHC-P: Human U87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RHOB antibody (ab155149)Anti-RHOB antibody (ab155149) at 1/500 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Predicted band size: 22 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHOB antibody (ab155149)Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue staining RHOB protein at cytosol with ab155149 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Western blot - Anti-RHOB antibody (ab155149)Anti-RHOB antibody (ab155149) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 22 kDa
12% SDS PAGE -
Western blot - Anti-RHOB antibody (ab155149)Anti-RHOB antibody (ab155149) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 22 kDa12% SDS-PAGE
