Anti-RHOB antibody [19HCLC] (ab277779)
![Anti-RHOB antibody [19HCLC] (ab277779)](https://www.abcam.com/ps/products/277/ab277779/Images/ab277779-446376-antiRhoBrecombinantpolyclonalantibody19hclcIF.jpg "Anti-RHOB antibody [19HCLC] (ab277779)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [19HCLC] to RHOB
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-RHOB antibody [19HCLC]
See all RHOB primary antibodies -
Description
Rabbit recombinant multiclonal [19HCLC] to RHOB -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RHOB aa 100-200.
Database link: P62745 -
Positive control
- ICC: A549 (human lung carcinoma cell line) cells. WB: HeLa, A549, COLO 205, SH-SY5Y, Mouse Brain and Rat Brain whole cell extract.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
19HCLC -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-RHOB antibody (ab277779)
For immunofluorescence analysis, UV (302 nm, 60 minutes) treated A549 (human lung carcinoma cell line) cells were fixed and permeabilized for detection of endogenous RhoB using Anti-RhoB Recombinant Rabbit Multiclonal Antibody (ab277779, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of RhoB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of RhoB. Panel e) shows untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Western blot - Anti-RHOB antibody (ab277779)All lanes : Anti-RHOB antibody [19HCLC] (ab277779) at 2 µg/ml
Lane 1 : HeLa whole cell extracts
Lane 2 : A549 whole cell extracts
Lane 3 : COLO 205 whole cell extracts
Lane 4 : SH-SY5Y whole cell extracts
Lane 5 : Mouse Brain whole cell extracts
Lane 6 : Rat Brain whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal, 0.4 µg/mL at 1/2500 dilution
Predicted band size: 22 kDa
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![Anti-RHOB antibody [19HCLC] (ab277779)](https://www.abcam.com/ps/products/277/ab277779/Images/ab277779-3-benefits-of-recombinant-antibodies.jpg)
Anti-RHOB antibody [19HCLC] (ab277779)