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Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5660] to ENAH/MENA - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free
    See all ENAH/MENA primary antibodies

  • Description

    Rabbit monoclonal [EPR5660] to ENAH/MENA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247959 is the carrier-free version of ab124685 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247959 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ENAH

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)
    This data was developed using ab124685, the same antibody clone in a different buffer formulation.ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)
    Western blot - Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)
    All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution

    Lane 1 : MDA-MB-435 (Human ductal carcinoma cell line) cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 67 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab124685, the same antibody clone in a different buffer formulation.

     

  • Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)
    Anti-ENAH/MENA antibody [EPR5660] - BSA and Azide free (ab247959)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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