Anti-RhD antibody [D7C2] - Chimeric (ab281911)
![Anti-RhD antibody [D7C2] - Chimeric (ab281911)](https://www.abcam.com/ps/products/281/ab281911/Images/ab281911-1-AntiRhD-antibody-immunohistochemistry-K562-cells.jpg "Anti-RhD antibody [D7C2] - Chimeric (ab281911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [D7C2] to RhD - Chimeric
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-RhD antibody [D7C2] - Chimeric
See all RhD primary antibodies -
Description
Rabbit monoclonal [D7C2] to RhD - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to RhD. Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r). Isolated peripheral lymphocytes were transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
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Positive control
- ICC: K562 cells.
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General notes
ab281911 is the chimeric rabbit version of ab281912.
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D7C2 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Images
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Immunocytochemistry - Anti-RhD antibody [D7C2] (ab281911)
Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 µg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.